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ATCC phoenix 293t packaging cells
Phoenix 293t Packaging Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Embryonic Kidney 293t Phoenix Eco Ecotropic Packaging Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC packaging 293t phoenix amphotropic cell line
( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in <t>HEK-293T</t> cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Packaging 293t Phoenix Amphotropic Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenHunter Corporation 293t phoenix packaging cell line
( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in <t>HEK-293T</t> cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
293t Phoenix Packaging Cell Line, supplied by GenHunter Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC phoenix eco 293t packaging cell line
( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in <t>HEK-293T</t> cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Phoenix Eco 293t Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC 293t phoenix ecotropic packaging cell line
( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in <t>HEK-293T</t> cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
293t Phoenix Ecotropic Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene human phoenix packaging cells (293t cells)
( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in <t>HEK-293T</t> cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.
Human Phoenix Packaging Cells (293t Cells), supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in HEK-293T cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

Journal: Scientific Reports

Article Title: Notch-regulated miR-223 targets the aryl hydrocarbon receptor pathway and increases cytokine production in macrophages from rheumatoid arthritis patients

doi: 10.1038/srep20223

Figure Lengend Snippet: ( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in HEK-293T cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

Article Snippet: Individual retroviral constructs were transfected into the packaging 293T Phoenix-Amphotropic cell line (provided by G. Nolan, Stanford University, Stanford, CA, USA), and supernatants obtained from puromycin-selected transfected cells were used to transduce OP-9 cells (ATCC) by centrifugation in the presence of polybrene.

Techniques: Expressing, Isolation, Cell Culture, Co-Culture Assay, FACS, Quantitative RT-PCR, MANN-WHITNEY